Isolation of Keratin from Waste Wool Using Hydrothermal Processes

The subcritical water (SubCW) extractions of waste wool to produce keratin were performed at temperatures 150 °C 250 and different reaction times between 5 min 75 min. resulting proteins in the obtained products confirmed with Fourier-transform infrared spectroscopy (FTIR). molecular weight protein extracts was determined by using two methods: a polyacrylamide gel electrophoresis presence sodium dodecyl sulphate (SDS-PAGE) permeation chromatography. results show, that SubCW, can be isolated from very high yields, much higher than other chemical methods. Maximal yield achieved 180 60 it 90.3%. distributions extracted proteins, which generated 14 kDa 4 kDa, what is comparable